Moreover, the concentrations of acetate, isobutyrate, isovalerate, valerate, and total SCFAs in cecum of birds fed AOS were significantly higher than the control birds ( P < 0.05). AOS also increased serum insulin-like growth factor-1, ghrelin ( P < 0.05), and growth hormone ( P < 0.1). AOS ameliorated the intestinal morphology, absorption function and barrier function, as indicated by the enhanced ( P < 0.05) intestinal villus height, maltase activity, and the expression of PEPT, SGLT1, ZNT1, and occludin. The results showed that dietary supplementation of 200 mg/kg AOS displayed the highest activity in promoting the birds’ ADG and ADFI ( P < 0.05). Animal trials were carried out using 320 1-day-old male Arbor Acres broilers (four groups 8 replicates/group × 10 chicks/replicate) receiving either a basal diet or the same diet supplemented with 100, 200 and 400 mg/kg PDE9-prepared AOS for 42 d. Resultsįive alginate lyases from bacteria were cloned into Pichia pastoris GS115 and the alginate lyase PDE9 was expressed at relatively high yield, activity and stability in P. This study aimed to optimize the enzymatic preparation of AOS by using bacterial alginate lyases expressed in yeast, investigate the effects of the prepared AOS on the growth performance and gut health of broiler chickens, and reveal the underlying mechanisms.
However, the effects of AOS on chicken health and the underlying mechanisms are not fully understood. Alginate oligosaccharide (AOS) holds great potential as a novel feed supplement in farm animals.
0 kommentar(er)
